nextRAD genotyping by sequencing samples a genome at thousands of loci across the genome. Each locus is sequenced with 122 bp reads and these reads are compared between samples to identify SNPs. A reference genome is not required for nextRAD genotyping.
Project pricing includes quality check of genomic DNA, creation of nextRAD libraries, sequencing, and bioinformatics (creation of genotype table). Extra charges apply for genome sizes greater than 1 gigabase.
95 samples
190 samples
380 samples
7,000 loci
single-end 122 bp reads at an average 20X read depth
not available
$55/sample
$50/sample
15,000 loci
single-end 122 bp reads at an average 20X read depth
$69/sample
$66/sample
$61/sample
30,000 loci
single-end 122 bp reads at an average 20X read depth
$84/sample
$81/sample
$76/sample